GTA: The Criminal Enterprises - The Loop
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Transcript: We put our eldest Sister through a new regimen of physical and mental conditioning, and suited her up with modified Protector equipment of my design. The younger ones have dubbed her a "Big Sister." Sadly, this is a stopgap. Between rogue Splicers and the aging of the girls, we'll run out of viable Little Sisters soon. After that... the surface may be our only source...